Two types of growth inhibitor in rat platelets for primary cultured rat hepatocytes |
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| Authors: | T Nakamura H Teramoto Y Tomita A Ichihara |
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| Institution: | 1. Citrus Research and Education Center, University of Florida, IFAS, 700 Experiment Station Road, Lake Alfred, FL 33850, USA;2. Gulf Coast Research and Education Center, University of Florida, IFAS, 14625 County Road, Wimauma, FL 33598, USA;3. Plant Protection Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt |
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| Abstract: | Rat platelets contain two types of growth inhibitor of adult rat hepatocytes in primary culture. One, named platelet derived growth inhibitor (PDGI)-alpha, is a heat- and acid-labile protein with a molecular weight of over 200 KD that is not released on thrombin treatment. The other, named PDGI-beta, is a heat- and acid-stable factor with a molecular weight of 24 KD that is released by thrombin. Both PDGI-alpha and -beta were inactivated by treatment with dithiothreitol. They both caused dose-dependent inhibition of DNA synthesis stimulated by insulin plus epidermal growth factor. These inhibitions were closely correlated with marked decrease in the labeling index. Neither PDGI-alpha nor -beta had a cytotoxic effect as judged by phase-contrast microscopic examination of the cells nor inhibition of protein synthesis. The properties of PDGI-beta suggest that it may be identical with transforming growth factor-beta. These results indicate that rat platelets contain not only a growth factor (HGF), but also growth inhibitors that affect adult rat hepatocytes. |
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