| 蛋白质错误折叠循环扩增及其在朊病毒蛋白检测中的应用研究进展 |
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| 引用本文： | 王海霞,石国庆,刘怡孝,万鹏程,管峰.蛋白质错误折叠循环扩增及其在朊病毒蛋白检测中的应用研究进展[J].细胞生物学杂志,2012(11):1141-1146. |
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| 作者姓名： | 王海霞 石国庆 刘怡孝 万鹏程 管峰 |
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| 作者单位： | [1]中国计量学院生命科学学院,杭州310018 [2]新疆兵团绵羊繁育生物技术重点实验室,石河子832000 [3]中国计量学院,浙江省生物计量及检验检疫技术重点实验室,植物环境生物学与检测技术研究所,杭州310018 |
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| 基金项目： | 国家高技术研究发展计划（863）（No.2008AA101011）; 国家自然科学基金（No.C120103）资助项目~~ |
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| 摘 要： | 朊病毒蛋白（prion protein,PrP）是传染性海绵状脑病的病原体,其检测是该病诊断的重要依据。该文从原理、方法、影响因素和检测应用方面对蛋白质错误折叠循环扩增（protein mis-folding cyclic amplification,PMCA）这种朊病毒蛋白新型检测技术做了介绍,旨在为朊病毒蛋白的检测和发病机制研究提供理论参考。
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| 关 键 词： | 朊病毒蛋白（PrP） PrP^C PrP^Sc 蛋白质错误折叠循环扩增（PMCA） 检测 |
Progress on Protein Misfolding Cyclic Amplification and Its Applications in the Detection of Prion Protein |
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| Wang Haixia,Shi Guoqing,Liu Yixiao,Wan Pengcheng,Guan Feng.Progress on Protein Misfolding Cyclic Amplification and Its Applications in the Detection of Prion Protein[J].Chinese Journal of Cell Biology,2012(11):1141-1146. |
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| Authors: | Wang Haixia Shi Guoqing Liu Yixiao Wan Pengcheng Guan Feng |
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| Institution: | (l College of Life Sciences, China Jiliang University, Hangzhou 310018, China; 2The Key Sheep Breeding and Reproduction Biotechnology Laboratory of Xinjiang Production and Construction Group, Shihezi 832000, China; 3China Jiliang University, Zhejiang Provincial Key Laboratory of Biometrology and lnspection ＆ Quarantine, Institute of Plant Environmental Biology and Measurement Technology, Hangzhou 310018, China) |
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| Abstract: | Prion protein （PrP） is the pathogen of transmissible spongiform encephalopathy （TSE）, and its detection is the important diagnosis basis of the disease. In this paper, a new technology named protein misfolding cyclic amplification was reviewed, including the technical principles, methods, influence factors and applications in the prion diseases detection. The aim is to provide theoretical references for the detection of prion protein and the mechanism of the disease. |
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| Keywords: | prion protein PrP^C PrP^Sc PMCA detection |
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