| 培养条件对棘孢曲霉SM-L22所产纤维素酶系组成的影响 |
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| 引用本文： | 杜娟,陈冠军,高培基. 培养条件对棘孢曲霉SM-L22所产纤维素酶系组成的影响[J]. 菌物学报, 2001, 20(4): 566-571 |
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| 作者姓名： | 杜娟 陈冠军 高培基 |
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| 作者单位： | 山东大学微生物技术国家重点实验室 济南250100(杜娟，陈冠军)，山东大学微生物技术国家重点实验室 济南250100(高培基) |
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| 基金项目： | 山东大学微生物技术国家重点实验室开放项目资助 |
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| 摘 要： | 本文研究了影响棘孢曲霉SM-L22纤维素酶系组成的培养条件。研究结果表明,碳源、氮源和初始pH对棘孢曲霉所产生纤维素酶的内、外切酶组分的比例有明显的影响。在2%麸皮,1%CF11,0.5%尿素或含尿素的复合氮源为氮源,初始pH为4.5时,28℃培养120h后,内、外切酶的比值最大,内切酶活可达到3.1 IU/ml,FPA为0.105 IU/ml,CMCase/FPase的比值为30.6。
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| 关 键 词： | 棘孢曲霉 纤维素酶 酶系组成 培养条件 |
| 文章编号： | 1007-3515(2001)04-0566-06 |
| 修稿时间： | 2000-09-01 |
EFFECTS OF FERMENTATION CONDITIONS ON ASPERGILLUS ACULEATUS SM-L22 CELLULASE COMPOSITION |
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| DU Juan,CHEN Guan-Jun,GAO Pei-Ji. EFFECTS OF FERMENTATION CONDITIONS ON ASPERGILLUS ACULEATUS SM-L22 CELLULASE COMPOSITION[J]. Mycosystema, 2001, 20(4): 566-571 |
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| Authors: | DU Juan CHEN Guan-Jun GAO Pei-Ji |
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| Abstract: | The effects of fermentation conditions on cellulase composition of Aspergillus aculeatus SM-L22 were studied. The results demonstrated that the carbon sources, nitrogen sources and the initial pH of the media can effect the rate of proportion of CMCase/FPase. The highest proportion of CMCase/FPase was obtained after growing 120 hr under the following culture conditions: 2% wheat bran, 1% cellulose CF11, 0.5% urea or mixed nitrogen resource containing urea and the initial pH of media being 4.5. When endoglucanase (CMCase) activity in the fermented liquid was 3.1 IU/ml and the FPase activity was 0.105 IU/ml, the final CMCase/FPase value reashed 30.6. |
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| Keywords: | Aspergillus aculeatus cellulase enzyme constitution culture conditions |
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