An automatic,on-line glucose analyzer for feed-back control of fed-batch growth of Escherichia coli |
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| Authors: | Gregory W Luli Steven M Schlasner Donald E Ordaz Marc Mason William R Strohl |
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| Institution: | (1) Department of Microbiology, The Ohio State University, 43210 Columbus, OH, USA;(2) Department of Chemical Engineering, The Ohio State University, 43210 Columbus, OH, USA;(3) YSI, Inc., 45387 Yellow Springs, OH, USA |
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| Abstract: | Summary A computer-assisted on-line glucose analyzer was developed for feed-back control of cell growth. Using this system the glucose consumption rate for Escherichia coli was determined to be linear during batch culture at 0.37 g/hr. On-line feed-back control of glucose concentration at 1.5±0.5 g/L was used with fed-batch cultures to produce 31.2 g dry weight of E. coli cells/L in 12 h. |
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