Effects of carbon concentration and carbon-to-nitrogen ratio on growth, conidiation, spore germination and efficacy of the potential bioherbicide Colletotrichum coccodes |
| |
Authors: | X Yu S G Hallett J Sheppard A K Watson |
| |
Institution: | (1) Department of Plant Science, Macdonald Campus of McGill University, Ste-Anne-de-Bellevue, Québec, Canada H9X 3V9, CA;(2) Department of Agricultural and Biosystems Engineering, Macdonald Campus of McGill University, Ste-Anne-de-Bellevue, Québec, Canada H9X 3V9, CA |
| |
Abstract: | The effect of carbon concentration and carbon-to-nitrogen ratio (C:N) as well as their interaction on Colletotrichum coccodes growth and sporulation in submerged flask culture were evaluated. When C:N ratios were held constant, both mycelial dry biomass
and spore yield increased with increasing carbon concentration. The specific spore yields (spore yield g−1 carbon), however, were not significantly different for the same C:N ratio in most cases. The highest spore yields (1.3 ×
108 spores per ml) were obtained from media containing 20 g per liter carbon with C:N ratios ranging from 5:1 to 10:1. When the
C:N ratio was greater than 15:1, spore yields were significantly decreased with increasing C:N ratios. High carbon concentration
(20 g L−1) combined with high C:N ratios (above 15:1) reduced both mycelial growth and sporulation, and increased spore matrix production.
Spores produced in medium containing 10 g L−1 carbon with C:N ratios from 10:1 to 15:1 had 90% germination on potato dextrose agar after 12 h and caused extensive shoot
dry weight reduction on the target weed, velvetleaf. These results suggest that C:N ratios from 10:1 to 15:1 are optimal for
C. coccodes spore production.
Received 9 December 1997/ Accepted in revised form 22 May 1998 |
| |
Keywords: | : carbon concentration Colletotrichum coccodes conidiation C:N ratio mycoherbicide |
本文献已被 SpringerLink 等数据库收录! |
|