Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects |
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Authors: | Wiśniewska Monika Nilsson Hans Olof Bak-Romaniszyn Leokadia Rechciński Tomasz Bielański Władysław Płaneta-Małecka Izabela Płonka Małgorzata Konturek Stanisław Wadström Torkel Rudnicka Wiesława Chmiela Magdalena |
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Institution: | Department of Immunology and Infectious Biology, University of Lód?, 90-237 Lód?, Banacha 12, Poland. |
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Abstract: | In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of H. pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection. |
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Keywords: | helicobacter pylori diagnosis stool antigens PCR |
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