Validation of an HPLC Analytical Method Coupled to a Multifunctional Clean-up Column for the Determination of Deoxynivalenol |
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| Authors: | Yoshiko Sugita-Konsihi Toshitsugu Tanaka Setsuko Tabata Masahiro Nakajima Masanori Nouno Yoko Nakaie Takao Chonan Mitsutoshi Aoyagi Nobuyuki Kibune Kazutoshi Mizuno Eiichi Ishikuro Naoki Kanamaru Masatoshi Minamisawa Norio Aita Masayo Kushiro Kenji Tanaka Kosuke Takatori |
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| Affiliation: | (1) National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan;(2) Kobe Institute of Health, Kobe, Japan;(3) Tokyo Metropolitan Institute of Public Health, Tokyo, Japan;(4) Nagoya City Public Health Research Institute, Nagoya, Japan;(5) Yokohama Quarantine Station, Yokohama, Japan;(6) Kobe Quarantine Station, Kobe, Japan;(7) Hokkaido Institute of Public Health, Hokkaido, Japan;(8) Japan Food Research Laboratories, Osaka branch, Osaka, Japan;(9) Fertilizer and Feed Inspection Services, Tokyo, Japan;(10) Japan Grain Inspection Association, Tokyo, Japan;(11) Fertilizer and Feed Inspection Services, Sendai Branch, Sendai, Japan;(12) National Food Research Institute, Tokyo, Japan |
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| Abstract: | To evaluate a method using a multifunctional clean-up column coupled with high performance liquid chromatography as an official analytical method for the determination of deoxynivalenol in wheat used as food or feed, an inter-laboratory study was performed in 12 laboratories using four naturally contaminated wheat samples and one spiked sample. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDR) of naturally contaminated wheat were in the range 5.8–11.3% and 12.0–20.7%, respectively. The HORRAT was less than 1.0 in each sample. From the spiking test, the recovery rate, RSDr, RSDR and HORRAT value were 100.0%, 11.2%, 10.3% and 0.5, respectively. The limit of quantification is 0.10 mg/kg from the range obtained in a linear calibration. Thus, it should be useful as a sensitive and validated analytical method for the determination of deoxynivalenol in wheat intended for use in food and feed. |
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| Keywords: | Deoxynivalenol (DON) HPLC inter-laboratory study multifunctional clean-up column validation wheat |
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