Centrilobular expression of ethanol-inducible cytochrome P-450 (IIE1) in rat liver |
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Authors: | M Ingelman-Sundberg I Johansson K E Penttil? H Glaumann K O Lindros |
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Institution: | Department of Physiological Chemistry, Karolinska Institute, Stockholm, Sweden. |
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Abstract: | Western blot analysis of digitonin eluates as well as immunohistochemical analysis revealed a 30-fold higher concentration of cytochrome P-450IIE1 in the centrilobular than in the periportal regions of the rat liver. Ethanol treatment caused a selective centrilobular induction of P-450IIE1, whereas phenobarbital induced P-450IIB1/2 in both liver lobule regions. The heterogeneous distribution pattern of P-450IIE1 was also observed in cells isolated from either region and correlated to the relative content of P-450IIE1 mRNA in the two cell types. The regiospecific expression and induction of P-450IIE1 may explain why several hepatotoxins, known to be metabolized by this isozyme, primarily damage the centrilobular region in the liver. |
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