Antifungal Susceptibility,Morphological and Molecular Characterization of <Emphasis Type="Italic">Lasiodiplodia theobromae</Emphasis> Isolated from a Patient with Keratitis |
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| Authors: | Priscila Dallé da Rosa Claudete Locatelli Karla Scheid Diane Marinho Lúcia Kliemann Alexandre Fuentefria Luciano Zubaran Goldani |
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| Institution: | 1.Programa de Pós-Gradua??o em Medicina: Ciências Médicas,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil;2.Servi?o de Oftalmologia, Hospital de Clínicas de Porto Alegre,Porto Alegre,Brazil;3.Departamento de Patologia da Faculdade de Medicina,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil;4.Programa de Pós-Gradua??o em Ciências Farmacêuticas,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil;5.Infectious Diseases Unit, Hospital de Clínicas de Porto Alegre,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil |
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| Abstract: | Lasiodiplodia theobromae is a rare ocular pathogen. We report a patient with fungal keratitis caused by L. theobromae. The patient was a 75-year-old male, a farmer with diabetes type II, and no previous history of ocular trauma. Histopathology analysis revealed the presence fungi invading Descemet’s membrane of the cornea. The fungus was characterized by septate, highly bulged fungal filaments involving full corneal thickness in the corresponding histopathology specimens. A dematiaceous mold was isolated and initally identified as L. theobromae by microscopic and macroscopic morphology, and further confirmed by PCR-based determination of internal transcribed spacer (ITS) regions of ribosomal DNA. Antifungal susceptibility tests showed sensitivity to amphotericin B (AMB) and voriconazole ( VRC), and resistance to other azoles, including itraconazole (ITC) and fluconazole (FLC). Corneal transplant was performed. Despite in vitro itraconazole resistance, the patient was successfully treated with oral itraconazole, topical voriconazole and natamycin, combined with ocular injections of amphotericin B and voriconazole. |
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