Toxoplasma gondii: Calcium lonophore A23187-mediated exit of trophozoites from infected murine macrophages |
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Authors: | T. Endo K.K. Sethi G. Piekarski |
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Affiliation: | 1. Institut für Medizinische Parasitologie, Universität Bonn, Sigmund Freud Strasse 25, D-5300 Bonn 1. West Germany;2. Institut für Medizinische Mikrobiologie und Immunologie, Universität Bonn, Sigmund Freud Strasse 25, D-5300 Bonn I. West Germany |
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Abstract: | The Ca2+ ionophore A23187 consistently induced the exit of Toxoplasma gondii trophozoites from cultured macrophages which they had recently infected. Following exit of toxoplasmas, the host macrophages underwent degeneration. A23187 was active at concentrations higher than 0.25 μM and the activity reached a plateau at the concentration of 1.0 μM. Noninfected macrophages or those engulfing heat-killed toxoplasmas, or some other particles, were not affected by treatment with A23187. The toxoplasmas exiting host cells were capable of infecting and proliferating in normal macrophages. The A23187-mediated exit of toxoplasmas proceeded despite external Ca2+ and was enhanced by the addition of ethylene glycol bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic acid (EGTA) in the reaction mixture. On the other hand, the A23187-mediated exit of toxoplasmas was inhibited significantly by exogenous Mg2+. |
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Keywords: | Protozoa, parasitic Trophozoite Mouse macrophage, cultured Ionophore Divalent cations Host-cell destructions Electron microscopy |
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