Plasmodium chabaudi: Antigenic variation during recrudescent parasitaemias in mice |
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Authors: | SA McLean CD Pearson RS Phillips |
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Institution: | Department of Zoology, University of Glasgow, Glasgow, G12 8QQ, Scotland United Kingdom |
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Abstract: | The A/S strain of Plasmodium chabaudi at different times was twice mosquito passaged and cloned by limiting dilution. Large groups of NIH mice were infected with 105 parasitized red cells of populations of parasites which were considered to be identical or very similar to the population forming the first erythrocytic parasitaemia seen in mice after mosquito transmission of the parasite. Most of the mice were killed immediately after the first patent parasitaemia had become subpatent and their sera pooled. The parasitaemias of surviving mice were followed until recrudescences appeared. The protective activity of the immune serum was then tested against the original infecting population and recrudescent populations by passive transfer tests in naive mice. Protection was measured as a delay in patent parasitaemia reaching 2% compared with normal serum recipients. The immune serum significantly delayed the 2% parasitaemia but in different experiments six out of seven recrudescent populations were found to be less sensitive to the effects of the immune serum than the original infecting population. The recrudescent populations retained their reduced or total insensitivity to the action of the immune serum after two blood passages and after eryopreservation. It appears, therefore, that P. chabaudi can undergo antigenic variation. |
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Keywords: | Protozoa parasitic Malaria murine Mosquito transmission NIH mice Passive transfer Recrudescences Antigenic variation |
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