Giardia spp.: Distribution of contractile proteins in the attachment organelle |
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Authors: | Dennis E. Feely Judy V. Schollmeyer Stanley L. Erlandsen |
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Affiliation: | Department of Anatomy, University of Minnesota, Minneapolis, Minnesota 55455, U.S.A. |
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Abstract: | Giardia spp. trophozoites isolated from rat small intestine were examined by light microscopy, electron microscopy, SDS-gel electrophoresis, and immunocytochemistry. In SDS-gels of protein extracts of isolated Giardia spp. trophozoites protein bands corresponding to myosin, α-actinin, and actin were identified by comigration with avian myofibril proteins and molecular weight standards. Actin was specifically identified in SDS-gels by immunoautoradiography. Immunostaining for actin, α-actinin, myosin, and tropomyosin in trophozoites was demonstrated in the periphery of the ventral disc in an area corresponding to the lateral crest. Electron-dense fibrillar was observed in the lateral crest of the ventral disc by electron microscopy. Immunostaining for actin and α-actinin was also observed in the area of the median body, a microtubular organelle, and in electron-dense fibrillar material associated with the intracellular axonemes of the posterior-lateral flagella. The localization of these contractile proteins in the ventral disc suggests that they may play an important role in the mechanism of trophozoite attachment. |
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Keywords: | Protozoa, parasitic Attachment organelle Morphology Immunochemistry Biochemistry Proteins, contractile |
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