Resistance Mechanism in a Terbinafine-Resistant Strain of <Emphasis Type="Italic">Microsporum canis</Emphasis> |
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| Authors: | Rui Kano Yun-Hsia Hsiao Hock Siew Han Charles Chen Atsuhiko Hasegawa Hiroshi Kamata |
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| Institution: | 1.Department of Veterinary Pathobiology,Nihon University College of Bioresource Sciences,Fujisawa,Japan;2.Asian Veterinary Specialist Referral Center,Taipei,Taiwan;3.The Animal Clinic,Singapore,Singapore;4.Teikyo University Institute of Medical Mycology,Hachioji, Tokyo,Japan |
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| Abstract: | To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresponding proteins with antifungals and with the efflux blocker FK506. The expression of the PDR1, MDR1, MDR2 and MDR4 genes was 2–4 times higher in the TRF-resistant strain grown in the presence of 0.14 µg/mL of TRF than in TRF-susceptible strains cultured in the absence of TRF. The TRF-resistant strain exhibited MICs of > 32 µg/mL for TRF alone; this resistance was attenuated to an MIC of 8 µg/mL in the presence of FK506, indicating that the TRF inhibitory concentration index value was < 0.75. The additive effect of the efflux blocker FK506 on TRF resistance was detected in the TRF-resistant strain. These results indicated that the TRF resistance in this strain reflects overexpression of genes encoding ABC transporter proteins. |
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