Specific conjugation of DNA binding proteins to DNA templates through thiol-disulfide exchange |
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| Authors: | Metelev Valeri G Kubareva Elena A Vorob'eva Olga V Romanenkov Andrey S Oretskaya Tatiana S |
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| Institution: | Chemical Department, M V Lomonosov Moscow State University, Vorob'evi gori, Moscow 119992, Russia. |
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| Abstract: | The double-stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficient conjugation of DNA methyltransferase SsoII to sequence-specific decoys was demonstrated. The obtained results assume that synthetic oligodeoxyribonucleotides bearing a new trapping site can be used as new tools to study and manipulate biological systems. |
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