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Atomic force microscopy detection of serological markers of viral hepatites B and C |
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| Authors: | Yu. D. Ivanov P. A. Frantsuzov T. O. Pleshakova V. S. Ziborov S. K. Svetlov N. V. Krohin V. A. Konev O. B. Kovalev V. F. Uchaikin O. N. Yastrebova P. G. Sveshnikov A. I. Archakov |
| Affiliation: | 1. Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russia 2. Scientific Association for High Temperatures, Russian Academy of Sciences, Moscow, Russia 3. JSV “VECTOR-BEST”, Novosibirsk, Russia 4. Russian Research Center of Molecular Diagnostics and Therapy, Moscow, Russia |
| Abstract: | The possibility of detection of serological markers, containing the hepatitis B surface antigen (HBsAg) and hepatitis C virus core-antigen (HCVcoreAg) in human serum, by a new atomic force microscopy (AFM)-based nanotechnological approach has been demonstrated. The antibodies against the hepatitis B virus surface antigen (anti-HBsAg) and the antibodies against the hepatitis C virus core antigen (anti-HCVcoreAg) were immobilized on an AFM-chip. It was shown that such approach enables to detect HBsAg, HCVcoreAg and the viral fragments containing these antigens in the serum. The comparative analysis of detection of HBsAg- and HCVcoreAg-containing particles by the AFM method versus traditional methods (ELISA, PCR) has demonstrated the 75% coincidence of results between the AFM and two other methods. |
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