Indocyanine green-enhanced multimodal photoacoustic microscopy and optical coherence tomography molecular imaging of choroidal neovascularization |
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| Authors: | Van Phuc Nguyen Jeff Folz Yanxiu Li Jessica Henry Wei Zhang Thomas Qian Xueding Wang Yannis M. Paulus |
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| Affiliation: | 1. Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, Michigan, USA;2. Biophysics Program, University of Michigan, Ann Arbor, Michigan, USA;3. Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA |
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| Abstract: | Photoacoustic microscopy (PAM) has great potential for visualization of the microvasculature with high spatial resolution and contrast. Early detection and differentiation of newly developed blood vessels named choroidal neovascularization (CNV) from normal vasculature remains a challenge in ophthalmology. Exogenous contrast agents can assist with improving PAM sensitivity, leading to differentiation of CNV. Here, an FDA-approved indocyanine green (ICG) was utilized as a PAM contrast agent. ICG was conjugated with RGD peptides, allowing the ICG to bind to the integrin expressed in CNV. Molecular PAM imaging showed that ICG-RGD can target CNV for up to 5 days post intravenous administration in living rabbits with a model of CNV. The PAM image sensitivity and image contrast were significantly enhanced by 15-fold at 24 h post-injection. Overall, the presented approach demonstrates the possibility of targeted ICG to be employed in PAM molecular imaging, allowing more precise evaluation of neovascularization. |
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| Keywords: | choroidal neovascularization contrast agent indocyanine green multimodal imaging modality optical coherence tomography photoacoustic microscopy |
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