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Optical plasticity of mammalian cells
Authors:Kaushikaram Subramanian  Heike Petzold  Benjamin Seelbinder  Lena Hersemann  Ina Nüsslein  Moritz Kreysing
Affiliation:1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany;2. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Center for Systems Biology Dresden, Dresden, Germany

Abstract:Transparency is widespread in nature, ranging from transparent insect wings to ocular tissues that enable you to read this text, and transparent marine vertebrates. And yet, cells and tissue models in biology are usually strongly light scattering and optically opaque, precluding deep optical microscopy. Here we describe the directed evolution of cultured mammalian cells toward increased transparency. We find that mutations greatly diversify the optical phenotype of Chinese Hamster Ovary cells, a cultured mammalian cell line. Furthermore, only three rounds of high-throughput optical selection and competitive growth are required to yield fit cells with greatly improved transparency. Based on 15 monoclonal cell lines derived from this directed evolution experiment, we find that the evolved transparency frequently goes along with a reduction of nuclear granularity and physiological shifts in gene expression profiles. In the future this optical plasticity of mammalian cells may facilitate genetic clearance of living tissues for in vivo microscopy.image
Keywords:biophotonics  light scattering  microscopy  nuclear architecture  optical clearing  photonics  tissue optics  tissue transparency
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