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Single-shot phase contrast microscopy using polarisation-resolved differential phase contrast |
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| Authors: | Ranjan Kalita William Flanagan Jonathan Lightley Sunil Kumar Yuriy Alexandrov Edwin Garcia Mark Hintze Michalis Barkoulas Chris Dunsby Paul M. W. French |
| Affiliation: | 1. Photonics Group, Physics Department, Imperial College London, London, UK;2. Photonics Group, Physics Department, Imperial College London, London, UK Francis Crick Institute, London, UK;3. Department of Life Sciences, Imperial College London, London, UK |
| Abstract: | We present a robust, low-cost single-shot implementation of differential phase microscopy utilising a polarisation-sensitive camera to simultaneously acquire four images from which phase contrast images can be calculated. This polarisation-resolved differential phase contrast (pDPC) microscopy technique can be easily integrated with fluorescence microscopy . |
| Keywords: | phase contrast microscopy polarisation-resolved imaging quantitative phase imaging |