| Affiliation: | 1. Internal Medicine V: Hematology & Oncology, Medical University of Innsbruck, Innsbruck, Austria;2. Institute of Pathology, Neuropathology and Molecular Pathology, Medical University of Innsbruck, Innsbruck, Austria;3. Material-Technology, Leopold-Franzens University Innsbruck, Innsbruck, Austria;4. Institute of Mineralogy and Petrography, Leopold-Franzens University Innsbruck, Innsbruck, Austria;5. Institute of Analytical Chemistry and Radiochemistry, Innsbruck, Austria;6. Internal Medicine V: Hematology & Oncology, Medical University of Innsbruck, Innsbruck, Austria Oncotyrol, Center for personalized Cancer Medicine, Innsbruck, Austria |
| Abstract: | Mid-infrared (MIR) microscopic imaging of indolent and aggressive lymphomas was performed including formalin-fixed and paraffin-embedded samples of six follicular lymphomas and 12 diffuse large B-cell-lymphomas as well as reactive lymph nodes to investigate benefits and challenges for lymphoma diagnosis. MIR images were compared to defined pathological characteristics such as indolent versus aggressive versus reactive, germinal centre versus activated cell-of-origin (COO) subtypes, or a low versus a high proliferative index and level of PD-L1 expression. We demonstrated that MIR microscopic imaging can differentiate between reactive lymph nodes, indolent and aggressive lymphoma samples. Also, it has potential to be used in the subtyping of lymphomas, as shown with the differentiation between COO subtypes, the level of proliferation and PD-L1 expression. MIR microscopic imaging is a promising tool for diagnosis and subtyping of lymphoma and further evaluation is needed to fully explore the advantages and disadvantages of this method for pathological diagnosis. |
