In vitro evaluation of photodynamic activity of methylene blue against Trichophyton verrucosum azole-susceptible and -resistant strains |
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| Authors: | Sebastian Gnat Dominik ?agowski Mariusz Dyl?g Jessica Zielinski Aneta Nowakiewicz |
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| Institution: | 1. Faculty of Veterinary Medicine, Department of Veterinary Microbiology, University of Life Sciences, Lublin, Poland;2. Faculty of Biological Sciences, Department of Mycology and Genetics, University of Wroclaw, Wroclaw, Poland;3. Hollings Cancer Center, Medical University of South Carolina, Charleston, South Carolina, USA |
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| Abstract: | The intense search for the “Holy Grail” of antifungal therapy can be observed today. The searches are not limited only to discovery of potential antifungal drugs, but also new therapeutic strategies involving the use of chemosensitizers to achieve synergistic effect or physicochemical factors inducing stress conditions in fungal cells. In this study was examined in vitro effectiveness of photodynamic antifungal strategy with methylene blue using a light beam with a wavelength equal to 635 nm toward the Trichophyton verrucosum susceptible and itraconazole- and/or fluconazole-resistant strains. Methylene blue used at concentration equal to 5 μg/mL and in the presence of 40 J/cm2 of light energy showed fungicidal effect toward the susceptible strains. However, for azole-resistant isolates, only the energy dose equal to 60 J/cm2 at 5 μg/mL of methylene blue allowed to kill the pathogen. This study confirms that methylene blue induced by red light has a definite inhibitory effect on zoophilic dermatophytes. |
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| Keywords: | methylene blue photodynamic therapy susceptibility Trichophyton verrucosum |
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