Measurement of cartilage sub-component distributions through the surface by Raman spectroscopy-based multivariate analysis |
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Authors: | Daniel Mason Sangeeta Murugkar Andrew D Speirs |
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Institution: | 1. Department of Mechanical and Aerospace Engineering, Carleton University, Ottawa, Canada;2. Department of Physics, Carleton University, Ottawa, Canada |
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Abstract: | Articular cartilage posesses unique material properties due to a complex depth-dependent composition of sub-components. Raman spectroscopy has proven valuable in quantifying this composition through cartilage cross-sections. However, cross-sectioning requires tissue destruction and is not practical in situ. In this work, Raman spectroscopy-based multivariate curve resolution (MCR) was employed in porcine cartilage samples (n = 12) to measure collagen, glycosaminoglycan, and water distributions through the surface for the first time; these were compared against cross-section standards. Through the surface Raman measurements proved reliable in predicting composition distribution up to a depth of approximately 0.5 mm. A fructose-based optical clearing agent (OCA) was also used in an attempt to further improve depth of resolution of this measurement method. However, it did not; mainly due to a high-spectral overlap with the Raman spectra of main cartilage sub-components. This measurement technique potentially could be used in situ, to better understand the etiology of joint diseases such as osteoarthritis (OA). |
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Keywords: | articular cartilage multivariate analysis optical clearing Raman spectroscopy |
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