Mapping fluorescence resonance energy transfer parameters of a bifunctional agent using time-domain fluorescence diffuse optical tomography |
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| Authors: | Weirong Mo Nayan J. Patel Yihui Chen Ravindra Pandey Ulas Sunar |
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| Affiliation: | 1. Topcon Healthcare Solutions, San Jose, California, USA;2. Department of Cell Stress Biology and PDT Center, Roswell Park Cancer Institute, Buffalo, New York, USA;3. Department of Biomedical Engineering, Wright State University, Dayton, Ohio, USA |
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| Abstract: | We present a method to map fluorescence resonance energy transfer (FRET) parameters of a bifunctional photodynamic therapy agent, (2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a)-cyanine dye (HPPH-CD) conjugate, which consists of a photosensitizer (HPPH) and a fluorescent agent CD. We utilized time-domain fluorescence diffuse optical tomography, the normalized Born ratio model in the Fourier-domain, and an iterative algorithm to map depth-resolved spatial heterogeneities of FRET parameters. Our results exhibited depth-resolved changes of fluorophore's lifetime and the distance maps due to FRET between HPPH and CD. Our model suggests a potential approach of using FRET parameters to monitor efficacies of multifunctional photodynamic therapy agents in deep tissue. |
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| Keywords: | diffuse optical tomography energy transfer fluorescence FRET imaging systems photodynamic therapy |
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