Dynamic imaging of zebrafish heart with multi-planar light sheet microscopy |
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| Authors: | Zengxin Huang Panchun Gu Dengfeng Kuang Ping Mi Xizeng Feng |
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| Institution: | 1. Tianjin Key Laboratory of Micro-scale Optical Information Science and Technology, and Institute of Modern Optics, Nankai University, Tianjin, China;2. State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, China |
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| Abstract: | Light sheet fluorescence microscopy has become a research hotspot in biomedicine because of low phototoxicity, high speed, and high resolution. However, the conventional methods to acquire three-dimensional spatial information are mainly based on scanning, which inevitably increases photodamage and is not real-time. Here, we propose a method to generate controllable multi-planar illumination with a dielectric isosceles triangular array and a design of multi-planar light sheet fluorescence microscopy system. We carry out experiments of three-dimensional illumination beam measurement, volumetric imaging of fluorescent microspheres, and dynamic in vivo imaging of zebrafish heart to evaluate the performance of this system. In addition, we apply this system to study the effects of bisphenol fluorene on the heart shape and heart-beating rate of zebrafish. Our experiment results indicate that the multi-planar light sheet microscopy system provides a novel and feasible method for three-dimensional selected plane imaging and low-phototoxicity in vivo imaging. |
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| Keywords: | biomedical imaging bisphenol fluorene light sheet fluorescence microscopy volumetric imaging illumination design zebrafish heart |
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