Follistatin allows efficient retroviral-mediated gene transfer into rat liver |
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Authors: | Borgnon Josephine Djamouri Fatima Lorand Isabelle Rico Virginie Di Loux Nathalie Pages Jean-Christophe Franco Dominique Capron Frédérique Weber Anne |
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Affiliation: | INSERM EMI 00-20 and University Paris XI, Bicêtre Hospital, Kremlin-Bicêtre, France. |
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Abstract: | Retroviral vectors are widely used tools for gene therapy. However, in vivo gene transfer is only effective in dividing cells, which, in liver, requires a regenerative stimulus. Follistatin is effective in promoting liver regeneration after 90% and 70% hepatectomy in rats. We studied its efficacy on liver regeneration and retroviral-mediated gene delivery in 50% hepatectomized rats. When human recombinant follistatin was infused into the portal vein immediately after 50% hepatectomy, hepatocyte proliferation was significantly higher than in control 50% hepatectomized rats. A single injection of virus particles administered 23 h after follistatin infusion resulted in more than 20% gene transduction efficiency in hepatocytes compared to 3% in control rats. It is concluded that a single injection of follistatin induces onset of proliferation in 50% hepatectomized rats and allows efficient retroviral-mediated gene transfer to the liver. |
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Keywords: | Follistatin Hepatectomy Liver regeneration Retroviruses In vivo gene transfer |
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