CBF 3 基因过量表达的拟南芥细胞质膜组分的变化

通过提取过量表达CBF3基因和对照的拟南芥Arabidopsis thaliana L．Heyn．(Columbia)]茎叶的质膜．分离并分析其脂类成分和蛋白质含量，从中探讨CBF3对膜脂成分的影响及与抗冷适应的关系。研究结果表明，过量表达CBF3植株的质膜膜脂总量和膜蛋白总量分别是对照的227％和190％，磷脂为105％，与冷适应诱导的效果相似。因此CBF3表达的变化可能对冷适应过程中质膜组成的改变起重要作用。

Changes of Components in Plasma Membrane of Arabidopsis Plants Overexpressed CBF3

The function of a cold response regulator gene TBCBF3 on membrane composition was studied. Plasma membrane from transgenic plant Arabidopsis Columbia constitutively expressing CBF3 gene and from control was extracted, membrane lipids and proteins were separated for further analysis. The results show that total lipids, total proteins and phospholipids of plasma membrane increased in constitutively expressing CBF3 transgenic plant by 227%, 190% for totoal lipids, and total proteins, by 105.05% for phophoslipids. This effect is similar to that of cold acclimation, indicates that CBF3 induce the changes of the components in plasma membrane during cold acclimation, and play an important role in this process.