Detection of Mineralized Structures in Early Stages of Development of Marine Teleostei Using a Modified Alcian Blue-Alizarin Red Double Staining Technique for Bone and Cartilage |
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Authors: | Paulo j Gavaia Carmen Sarasquete M Leonor Cancela |
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Institution: |
a Center for Marine Sciences. CCMar, University of Algarve, Faro, Portugal
b Institute of Marine Sciences of Andalucia, Spanish Council for Scientific Research, Puerto Real, Cadiz, Spain |
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Abstract: | We have developed a procedure for staining cartilage and bone in fish larvae as small as 2 mm (notochord length), for which standard alcian blue/alizarin red procedures did not give positive and/or consistent results. Small calcified structures only 100-200 ixm in length can be clearly visualized. The method is suitable for both onto-genic studies during early stages of skeletal development in most marine fishes (e.g., Sporus aurata L., Solea senegalensis Kaup), whose larvae at hatching are often only a few millimeters long and for detecting skeletal abnormalities in small larvae. This procedure can also be used for specimens that have been preserved in 100% ethanol for up to two years. |
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Keywords: | alcian blue alizarin red bone cartilage Diplodus sp Halobatrachus didactilns larvae skeleton Solea senegalensis Spams aurata |
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