Automated determination of 5-fluorouracil and its metabolite in urine by high-performance liquid chromatography with column switching |
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Authors: | Tohru Maeda Satoshi Sumi Katsuo Hayashi Kiyoshi Kidouchi Tadashi Owaki Hajime Togari Shinji Fujimoto Yoshiro Wada |
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Affiliation: | a Department of Hospital Pharmacy, Nagoya City University Hospital, 1 Kawasumi, Mizuho-ku, Nagoya 467-0001, Japan;b Department of Pediatrics, Nagoya City University Medical School, 1 Kawasumi, Mizuho-ku, Nagoya 467-0001, Japan;c Second Department of Internal Medicine, Nagoya City University Hospital, 1 Kawasumi, Mizuho-ku, Nagoya 467-0001, Japan;d Department of Pediatrics, Higashi Municipal Hospital of Nagoya, 1-2-23 Wakamizu, Chikusa-ku, Nagoya 464-0071, Japan;e Department of Internal Medicine, Higashi Municipal Hospital of Nagoya, 1-2-23 Wakamizu, Chikusa-ku, Nagoya 464-0071, Japan;f Department of Laboratory Medicine, Nagoya City University Medical School, 1 Kawasumi, Mizuho-ku, Nagoya 467-0001, Japan |
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Abstract: | We report a quantitative assay of 5-fluorouracil (FU) and its metabolite, 5-fluorodihydrouracil (FDHU) in human urine by used a column-switching high-performance liquid chromatographic method. The analyses were carried out using a molecular exclusion column for sample purification, and a cation-exchange column for separation. Each sample required only 40 min to analyze, and required no preparation other than filtration. Linearity was verified up to 1000 nmol/ml (r>0.993). The recovery of FU was 96–101%; recovery of FDHU was 96–105%. The imprecision (RSD) for FU (10–100 nmol/ml) was <1.5%, same-day (n=5), and <1.8%, day-to-day (n=5). The imprecision (RSD) for FDHU (10–100 nmol/ml) was <3.2%, same-day (n=5), and <4.0%, day-to-day (n=5). The detection limits were, respectively, 0.1 nmol/ml. We measured FU and FDHU in urine of seven cancer patients after oral administration of FU. The cumulative quantity ratio of the FDHU and FU (FDHU/FU) excreted in their urine within 120 min after FU administration was a constant value in all seven patients. Based on these results, we believe that our method provides a useful tool for evaluating FU metabolism. |
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Keywords: | 5-Fluorouracil 5-Fluorodihydrouracil |
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