Determination of ginsenoside Rg3 in plasma by solid-phase extraction and high-performance liquid chromatography for pharmacokinetic study |
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Authors: | Hailin Wang Hanfa Zou Liang Kong Yukui Zhang Huan Pang Chenye Su Guoyou Liu Ming Hui Li Fu |
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Affiliation: | a National Chromatographic R&A Center of China, Dalian Institute of Chemical Physics, Academia Sinica, Dalian 116011, China;b Department of Clinical Pharmacology, Dalian Medical University, Dalian 116023, China;c Dalian Tianfu Institute of Natural Medicine, Dalian 116600, China |
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Abstract: | A method using high-performance liquid chromatography (HPLC) and solid-phase extraction (SPE) is described for the determination of ginsenoside Rg3 in human plasma. A 2.5-ml volume of plasma was mixed with 2.5 ml 60% methanol aqueous solution, and centrifuged at 1100 g for 10 min, the supernatant fluid was further purified by SPE with 200 mg/5 ml 40 μm octadecyl silica and separation was obtained using a reversed-phase column under isocratic conditions with ultraviolet absorbance detection. The intra- and inter-day precision, determined as relative standard deviations, were less than 5.0%, and method recovery was more than 97%. The lower limit of quantitation, based on standards with acceptable RSDs, was 2.5 ng/ml. No endogenous compounds were found to interfere with analyte. A good linear relationship with a regression coefficient of 0.9999 in the range of 2.5 to 200 ng/ml was observed. This method has been demonstrated to be suitable for pharmacokinetic studies in humans. Method development for determination of drug with low UV absorption by SPE and HPLC is also discussed. |
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Keywords: | Ginsenosides |
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