| 高效表达N-乙酰高丝氨酸内酯酶-木聚糖酶融合蛋白及其酶学性质 |
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| 引用本文： | 张美超,曹雅男,姚斌,周志刚. 高效表达N-乙酰高丝氨酸内酯酶-木聚糖酶融合蛋白及其酶学性质[J]. 微生物学报, 2011, 51(8): 1052-1061 |
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| 作者姓名： | 张美超 曹雅男 姚斌 周志刚 |
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| 作者单位： | 中国农业科学院饲料研究所,农业部饲料生物技术重点试验室,北京100081 |
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| 基金项目： | 科技部农业科技成果转化资金项目(2010GB23260591); 中国农业科学院饲料研究所所长基金(2009);中国博士后科学基金(20090450472) |
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| 摘 要： | 摘要: 【目的】构建N-乙酰高丝氨酸内酯酶-木聚糖酶双酶活性表达毕氏酵母重组菌株，并对经纯化的重组蛋白SL2B 进行N-乙酰高丝氨酸内酯酶及木聚糖酶酶学性质的研究。【方法】利用PCR 拼接技术得到N-乙酰高丝氨酸内酯酶基因aiiA-B546 和木聚糖酶基因xynAS27cd 融合而成的基因Sl2b。构建重组表达载体pPIC9 / Sl2b 转化毕氏酵母，筛选得到同时具有木聚糖酶和N-乙酰高丝氨酸内酯酶活性的重组子，随后对经硫酸铵沉淀、分子筛纯化后得到的重组蛋白SL2B 进行N-乙酰高丝氨酸内酯酶及木聚糖酶
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| 关 键 词： | 关键词: 高丝氨酸内酯酶，木聚糖酶，融合表达，酶学性质 |
| 收稿时间： | 2012-02-22 |
| 修稿时间： | 2012-04-29 |
High expression and characterization of N-acyl-homoserine lactonase-xylanase recombinant protein |
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| Meichao Zhang,Yanan Cao,Bin Yao and Zhigang Zhou. High expression and characterization of N-acyl-homoserine lactonase-xylanase recombinant protein[J]. Acta microbiologica Sinica, 2011, 51(8): 1052-1061 |
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| Authors: | Meichao Zhang Yanan Cao Bin Yao Zhigang Zhou |
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| Affiliation: | Meichao Zhang,Yanan Cao,Bin Yao,Zhigang Zhou* Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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| Abstract: | [Objective] The fusion protein SL2B with both xylanase and N-acyl-homoserine lactonase activities was expressed in Pichia pastori.Characterization of the purified xylanase and N-acyl-homoserine lactonase fusion protein SL2B was investigated.[Methods] The fusion gene sl2b was amplified from the N-acyl-homoserine lactonase gene aiiA-B546 and the xylanase gene xynAS27cd via overlap PCR technique.After the recombinant vector pPIC9/sl2b was transformed into P.pastoris,transformants with both xylanase and N-acyl-... |
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| Keywords: | fusion expression enzymatic properties n-acyl-homoserine lactonase |
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