Quality control testing of surfaces for mammalian cell culture using cells propagated in a protein-free medium |
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Authors: | J Cinatl J Cinatl H Rabenau J W Ahn H W Doerr |
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Institution: | Centre of Hygiene, Department of Virology, Clinics of the J.W. Goethe University Frankfurt a. M., Federal Republic of Germany. |
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Abstract: | Mouse NCTC clone 929 L (L-929) cells were propagated continuously for 3 years as monolayers in a protein-free chemically-defined medium. These cells, designated L-929-WS, were used for quality control testing of the surfaces of commercially available cell culture plastic flasks. Differences in attachment and saturation density of L-929-WS cells in a protein-free culture medium were taken to define various levels of quality of the culture vessels tested. The rate of attachment and growth of L-929-WS cells on a surface of a given quality correlated directly with that of human embryonal fibroblasts and embryonal epithelial cells grown in a serum-free medium supplemented with growth factors and hormones. L-929-WS cells propagated continuously in a protein-free medium provide a simple and sensitive assay system for more general quality control testing of surfaces used for the culture of monolayer cells. |
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