Complete rescue of the nude mutant phenotype by a wild-type <Emphasis Type="BoldItalic">Foxn1</Emphasis> transgene |
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Authors: | Vincent T Cunliffe Andrew JW Furley Deborah Keenan |
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Institution: | (1) Centre for Developmental Genetics, School of Medicine and Biomedical Science, University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK, GB |
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Abstract: | In this paper we describe the production and analysis of mice carrying a 110-kb transgene that encompasses the wild-type
Foxn1 genomic locus. Mutations in Foxn1 cause the nude phenotype. We show that in the hair follicles, transgenic mice with increased Foxn1 gene dosage exhibited increased Foxn1 expression that was restricted correctly to the nascent, post-mitotic cells of the differentiating hair cortex and hair cuticle
lineages. We also demonstrate for the first time that a Foxn1 transgene rescues completely both the hair follicle and the thymus defects in animals that are also homozygous for the nude
mutation at the endogenous Foxn1 locus, causing the development of a full coat of hair and a normal population of peripheral blood T lymphocytes. We conclude
that sufficient cis-acting regulatory information resides within this 110-kb transgene to direct reliable and appropriate tissue-specific expression
of the Foxn1 gene. |
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