Analysis of chromosome aberrations on the basis of synaptonemal complexes in the offspring of mice irradiated with gamma-rays |
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Authors: | E I Kalikinskaia Iu F Bogdanov O L Kolomiets V A Shevchenko |
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Abstract: | Electron-microscopic analysis of synaptonemal complexes (SC) spread on the surface of hypophase was carried out to study chromosome rearrangements in sterile and semisterile F1 offsprings of mice exposed to gamma-radiation at a dose of 5 Gy. Chromosome rearrangements were microscopically scored at diakinesis - metaphase I in the same animals. SC analysis at pachytene revealed chromosome rearrangements in 63% spermatocytes. Analysis of chromosomes at diakinesis - metaphase I in the same animals only revealed chromosome rearrangements in 32% cells. SC analysis permits detecting chromosome rearrangements undetectable at diakinesis - metaphase I. |
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