感染囊幼病的工蜂咽下腺细胞的超微结构的变化

前言 中华蜜蜂囊幼病是我国养蜂业的一种重要病毒病,发病率很高,有的地区造成大量的幼虫死亡,给我国养蜂生产带来一定的危害,为了对防治病害提供科学的依据,我们首先对该病病原进行了分离、提纯及电子显微镜的研究等工作。发现该病毒对幼虫和成蜂,尤其对工蜂体内各个器官都有程度不同的影响。在幼虫发病期症状特别明显,病幼虫身体松软多水,其表皮容易破裂,当悬挂幼虫时其幼虫末端积聚有透明的液滴,在巢房内幼虫头部尖并变成黑色,头部稍微向上抬起呈船形。死后的幼虫干涸变为褐色的外壳留于巢房内,当感染的幼虫全部封盖后,巢房盖的中央有一小孔,这些异常的变化都是囊幼病的典型症状。幼虫症状虽很明显,但工蜂感染病毒后在外部形态上却没有明显

ULTRASTRUCTURAL CHANGE IN THE HYPOPHARYNGEAL GLANDS OF WORKER HONEY BEE (APIS CERANA) INFECTED WITH SACBROOD VIRUS

Secretion of royal jelly is an important function of the hypopharyngeal glands of worker honey bee. Royal jelly is used to feed honey bee larvae and queens.Hypopharyngeal glands of infected worker bees showed the area between the intracellular ductulcs and the outer layer membrane was greatly reduced in 5-6 days old infected worker bee as contrasted with that of the healthy bees. The reduction of the outer layer area of intracellular ductulcs in hypopharyngeal glands of sacbrood-infected bee suggested that royal jelly secreted was much less than in healthy bees of the same age.The gland of diseased bee showed the disappearance of the rough endo-plasmic rccticulum and fewer ribosomes were seen in the gland of diseased bee than in the glands of healthy bees.There were variations in special functional state of the cell. In infected bee, remnants of mitochondria were observed in hypopharyngeal glands cells, while the mitochondria of diseased bee were transformed into large vacuoles and enlarged lysosome transformed into myelinlikc membranes.