7-Deoxyloganin 7-hydroxylase in Lonicera japonica cell cultures |
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| Authors: | Katano N Yamamoto H Iio R Inoue K |
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| Institution: | Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan. achkawa@agr.shizuoka.ac.jp |
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| Abstract: | A lectin was isolated from the mushroom Mycoleptodonoides aitchisonii by means of affinity chromatography on bovine submaxillary mucin (BSM)-Toyopearl and gel filtration on Superose 12 HR10/30 using a FPLC system. This lectin is composed of four identical 16 kDa subunits and the molecular mass of the intact lectin was estimated to be 64 kDa by gel filtration. In a hemagglutination inhibition assay, it exhibited strong sugar-binding specificity towards asialo-BSM among the mono- or oligo-saccharides and glycoproteins tested. The binding specificity of the lectin was also examined by surface plasmon resonance analysis. |
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