| 圈卷产色链霉菌尼可霉素生物合成基因-sanK的克隆及功能研究 |
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| 引用本文： | 聂丽平,李文利,谭华荣. 圈卷产色链霉菌尼可霉素生物合成基因-sanK的克隆及功能研究[J]. 生物工程学报, 2000, 16(6): 667-670 |
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| 作者姓名： | 聂丽平 李文利 谭华荣 |
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| 作者单位： | 1. 中国科学院微生物研究所,北京,100080;辽宁师范大学生物系,大连,116029
2. 中国科学院微生物研究所,北京,100080 |
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| 基金项目： | 国家自然科学基金重点项目(39830010)及国家杰出青年科学基金(39925002)资助。 |
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| 摘 要： | 利用染色体步移策略，以尼可霉素生物合成相关的基因片段为探针，从圈卷产色链霉菌中克隆到了一个大约１０ｋｂ的ＤＮＡ片段。对其中１．８ｋｂ的ＰｖｕⅡ－ＳａｃⅡ片段进行了序列分析，结果表明：此片段中含有一个具有１１７０个核苷酸的完整开放阅读框，起始密码子为４４７位的ＡＴＧ，终止密码子为１６１４位的ＴＧＡ，推测其编码一个３８９个氨基酸的蛋白质产物。利用ＢＬＡＳＴＸ程序进行了分析揭示，此基因编码一个肌氨酸单体
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| 关 键 词： | 圈卷产色链霉菌 尼可霉素 生物合成 |
| 文章编号： | 1000-3061(2000)06-0667-04 |
| 修稿时间： | 2000-03-21 |
Cloning and Function of sanK-a Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes |
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| NIE Li-Ping,LI Wen-Li,TAN Hua-Rong. Cloning and Function of sanK-a Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes[J]. Chinese journal of biotechnology, 2000, 16(6): 667-670 |
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| Authors: | NIE Li-Ping LI Wen-Li TAN Hua-Rong |
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| Affiliation: | NIE Li-Ping;(Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080 Department of biology, Liaoning Normal university, Dalian 116029);LI Wen-Li,TAN Hua-Rong;(Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080) |
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| Abstract: | The chromosome walking strategy has been used in this experiment and a 10kb DNA fragment was cloned from the cosmid library of %Streptomyces ansochromogenes% using a partial DNA fragment involved in the nikkomycins biosynthesis as a probe.The nucleotide sequence of 1.8kb Pvu II| Sac II DNA fragment was determined and analysed.The result indicated that the 1170bp DNA fragment contains one complete open reading frame (ORF).The start codon is ATG at 447 bp position,and the stop codon is TGA at 1614bp position,the predicted product is a monomeric sarcosine oxadase using BLASTX programme.The experiment of gene disruption showed that the gene is closely related to nikkomycins biosynthesis of %Streptomyces ansochromogenes.%This gene was designated as %sanK.% |
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| Keywords: | Streptomyces ansochromogenes nikkomycin biosynthesis gene disruption monomeric sarcosine oxadase |
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