Opposite Effects of Mn(III) and Fe(III) Forms of meso-Tetrakis(4-N-methyl pyridiniumyl) Porphyrins on Isolated Rat Liver Mitochondria |
| |
Authors: | M F Nepomuceno M Tabak A E Vercesi |
| |
Institution: | (1) Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brazil;(2) Universidade de São Paulo, C. P. 780, 13560-970 São Carlos, SP, Brazil |
| |
Abstract: | The relevance of porphyrins as therapeutic drugs targeted to mitochondria has been widely recognized. In this work, we studied the action of meso-tetrakis porphyrins (TMPyP) on respiring rat liver mitochondria. Mn(III)TMPyP exerted a protective effect against lipid peroxidation induced by Fe(II) or the azo initiator 4,4 -azobis(4-cyanopentanoic acid) (ABCPA), which partition in the hydrophobic phospholipid moiety, and 2,2 -azobis(2-amidinepropane)dihydrochloride (ABAP), which partitions in the aqueous phase. In contrast, Fe(III)TMPyP itself induced an intense lipid peroxidation, accompanied by mitochondrial permeability transition. Both mesoporphyrins studied promoted a release of mitochondrial state-4 respiration, in the concentration range of 1.0–20 M. Based on the relative effects of Mn(III)TMPyP against ABAP and ABCPA-induced lipid peroxidation, we believe that meso-tetrakis porphyrins must concentrate preferably at membrane–water interfaces. |
| |
Keywords: | Oxygen consumption free radicals oxidative stress photodynamic therapy |
本文献已被 PubMed SpringerLink 等数据库收录! |
|