Production of a novel extracellular acidic lipase from Pseudomonas gessardii using slaughterhouse waste as a substrate |
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Authors: | K. Ramani Evvie Chockalingam G. Sekaran |
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Affiliation: | (1) Environmental Technology Division, Central Leather Research Institute (CSIR), Adyar, Chennai, 600 020, Tamilnadu, India; |
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Abstract: | An isolate exhibiting high extracellular lipolytic activity was identified as Pseudomonas gessardii by 16S rDNA gene sequence analysis. The slaughterhouse waste, goat tallow, was used as a lipid substrate for the production of acidic lipase by P. gessardii. The maximum lipase activity of 156 U/ml was observed at an acidic pH of 3.5 and at 0.31 g substrate concentration. The purification steps resulted in the isolation of acidic lipase with a specific activity of 1,473 U/mg and a molecular weight of 94 kDa. One interesting feature of this purified lipase is its stability at highly acidic pH ranging from 2.0 to 5.5 with a high molecular weight. The amino acid composition was determined using HPLC. This acidic lipase has potential applications in the medicinal field as a substitute for pancreatic lipases for enzyme therapy, oleochemical and in biotechnological industries. |
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