Glycolipids of cell surfaces: Isolation of specific sugar sequences using carbohydrate-binding proteins |
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Authors: | David F. Smith John L. Magnani Victor Ginsburg |
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Affiliation: | National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20205 U.S.A. |
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Abstract: | Proteins that bind carbohydrates can be used to isolate specific sugar sequences from complex mixtures. Free sialyloligosaccharides or sialyloligosaccharides released from gangliosides by ozonolysis and alkaline fragmentation are labeled at their reducing ends by reduction with NaB[3H]4. After partial separation by column chromatography, oligosaccharide fractions are tested for binding to anti-sialyloligosaccharide antibodies [Smith, D. F., and Ginsburg, V. (1980) J. Biol. Chem.255, 55–59] and cholera toxin by a nitrocellulose filter assay. Oligosaccharides bound by the proteins can be eluted from the filters and further characterized. The method can be used to isolate and identify carbohydrate ligands of cell surfaces. |
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