Subcellular localization of acetyl-CoA synthetase in leaf protoplasts of Spinacia oleracea |
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Authors: | David N Kuhn Michael Knauf PK Stumpf |
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Institution: | Department of Biochemistry & Biophysics, University of California, Davis, California 95616 U.S.A. |
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Abstract: | The localization of acetyl-CoA synthetase in the spinach leaf cell was examined. When the different compartments of lysed spinach protoplasts were assayed for marker enzymes and acetyl-CoA synthetase, it was determined that the synthetase was totally localized in the chloroplast compartment. Analysis of spinach leaf for free acetate revealed that this acid was present at a 1 mm level in the leaf cell. It is suggested that free acetate probably derived from a number of sources in the cell diffuses into the chloroplast stroma compartment where it is converted to acetyl-CoA and thence employed for biosynthetic reactions. Thus, free acetate is metabolically inert in the leaf cell until it is transported to the only compartment that contains acetyl-CoA synthetase, namely the chloroplast. |
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