Investigation of the mechanism of glyceollin accumulation in soybean infected by Phytophthora megasperma f. sp. glycinea |
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Authors: | Peter Moesta Hans Grisebach |
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Affiliation: | Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität Freiburg, Schänzlestrasse 1, D-7800 Freiburg im Breisgau, West Germany |
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Abstract: | Soybean seedlings (Glycine max, cv. Harosoy 63) which had been inoculated in the hypocotyls with mycelium from either race 1 (incompatible) or race 3 (compatible) of Phytophthora megasperma f. sp. glycinea were pulse labeled with 14CO2. The time course of accumulation of glyceollin and daidzein and of 14C incorporation into these compounds was determined. Metabolic rates of glyceollin were measured by pulse-chase experiments. Differences in glyceollin accumulation between the incompatible and compatible interaction were not apparent before about 14 h after inoculation. Subsequently glyceollin accumulated to a higher level in the incompatible interaction. This difference is also reflected in the rate of 14C incorporation, which declines more rapidly in the compatible interaction. The apparent half-life of glyceollin metabolism was 28 ± 7 h for inoculation with race 1, while no metabolism was observed with race 3. In contrast to a previous report (M. Yoshikawa, K. Yamauchi, and H. Masago (1979)Physiol. Plant Pathol.14, 157–169), our data prove that the higher accumulation of glyceollin in the incompatible interaction is due to a longer duration of synthetic activity and that the level of glyceollin in both the incompatible and compatible interaction is determined predominantly by its rate of synthesis. |
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