A specific inhibitor to δ-aminolevulinate dehydratase in rat bone marrow cells |
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| Authors: | Masao Kondo Masatoshi Kajimoto Hideko Kimura Taeko Suzuki Atsuko Sasaki Motoo Niwa Gumpei Urata |
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| Institution: | Department of Nutrition and Biochemistry, Institute of Public Health, Minato-ku, Tokyo 108, Japan |
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| Abstract: | A factor that specifically inhibited δ-aminolevulinate dehydratase was found in rat bone marrow cells. The inhibitor, which was located in the supernatant fraction of the bone marrow hemolysate, was purified about 12-fold by ammonium sulfate fractionation and column chromatography on Sephadex G-75. The partially purified inhibitor was heat labile and sensitive to trypsin and was denatured by urea. It had a pH optimum of 7.5–8.0, and a molecular weight of 28,000. It inhibited the activity of δ-aminolevulinate dehydratase noncompetitively. |
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| Keywords: | Author to whom reprint requests and correspondence should be addressed |
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