Replacement of nucleosomal histones by histone H1-like proteins during spermiogenesis in Cnidaria: Evolutionary implications |
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| Authors: | Corinne Rocchini Roswitha M Marx Joachim Schnorr von Carosfeld Harold E Kasinsky Ellen Rosenberg Freya Sommer Juan Ausio |
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| Institution: | (1) Department of Biochemistry and Microbiology, University of Victoria, V8W 3P6 Victoria, British Columbia, Canada;(2) Department of Zoology, University of British Columbia, V6T 1Z4 Vancouver, British Columbia, Canada;(3) Department of Botany, University of British Columbia, V6T 1Z4 Vancouver, British Columbia, Canada;(4) Monterey Bay Aquarium, 93940 Monterey, CA, USA |
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| Abstract: | We have analyzed the chromosomal protein composition of the sperm from several species belonging to three different classes (Hydrozoa, Scyphozoa, Anthozoa) of the phylum Cnidaria. In every instance, the sperm nuclear basic proteins (SNBPs) were found to consist of one to two major protein fractions that belong to the histone H1 family, as can be deduced from their amino acid composition and solubility in dilute perchloric acid, and the presence of a trypsin-resistant core. In those species where mature spawned sperm could be obtained, we were able to show that these proteins completely replace the somatic histones from the stem cells that are present at the onset of spermatogenesis. The presence of a highly specialized histone H1 molecule in the sperm of this phylum provides support for the idea that the protamine-like proteins (PL) from higher groups in the phylogenetic tree (and possibly protamines as well) may all have evolved from a primitive histone H1 ancestor. |
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| Keywords: | Sperm nuclear basic proteins (SNBPs) Histone H1 Cnidaria Evolution |
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