Molecular profiling of drug resistant isolates of Mycobacterium
tuberculosis in the state of Santa Catarina,southern
Brazil |
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Authors: | Rodrigo Ivan Prim Marcos André Sch?rner Simone Gon?alves Senna Christiane Louren?o Nogueira Anna Carolina Can?ado Figueiredo Jaquelline Germano de Oliveira Darcita Bürger Rovaris Maria Luiza Bazzo |
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Institution: | 1.Universidade Federal de Santa Catarina, Laboratório de Biologia Molecular, Sorologia e Micobactérias, Florianópolis, SC, Brasil;2.Fundação Oswaldo Cruz, Centro de Pesquisas René Rachou, Belo Horizonte, MG, Brasil;3.Laboratório Central de Saúde Pública de Santa Catarina, Florianópolis, SC, Brasil |
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Abstract: | Drug resistance is a global threat and one of the main contributing factors to
tuberculosis (TB) outbreaks. The goal of this study was to analyse the molecular
profile of multidrug-resistant TB (MDR-TB) in the state of Santa Catarina in southern
Brazil. Fifty-three MDR Mycobacterium tuberculosisclinical isolates
were analysed by spoligotyping and a partial region of therpoB gene,
which is associated with rifampicin resistance (RMP-R), was sequenced. Some isolates
were also distinguished by their mycobacterial interspersed repetitive units (MIRU).
S531L was the most prevalent mutation found within rpoB in RMP-R
isolates (58.5%), followed by S531W (20.8%). Only two MDR isolates showed no
mutations withinrpoB. Isolates of the Latin American Mediterranean
(LAM) family were the most prevalent (45.3%) found by spoligotyping, followed by
Haarlem (9.4%) and T (7.5%) families. SIT106 was found in 26.4% of isolates and all
SIT106 isolates typed by MIRU-12 (5 out of 14) belong to MIT251. There was a high
correlation between the S531W mutation and the LAM family mainly because all SIT2263
(LAM9) isolates carry this mutation. Among isolates with the S531W mutation in
rpoB MIRU demonstrates a cluster formed by four isolates (SIT2263
and MIT163) and very similar profiles were observed between eight of the nine
isolates. Better characterisation of TB isolates may lead to new ways in which to
control and treat TB in this region of Brazil. |
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Keywords: | tuberculosis MDR-TB rpoB spoligotyping MIRU |
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