Crop-to-crop Gene Flow using Farm Scale Sites of Oilseed Rape (<Emphasis Type="Italic">Brassica napus</Emphasis>) in the UK |
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Authors: | Rebecca?Weekes Carola?Deppe Email author" target="_blank">Theo?AllnuttEmail author Caroline?Boffey Derek?Morgan Sarah?Morgan Mark?Bilton Roger?Daniels Christine?Henry |
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Institution: | (1) Central Science Laboratory, YO41 1LZ Sand Hutton, York, UK;(2) Centre for Ecology and Hydrology, Winfrith Technology Centre, Winfrith Newburgh, DT2 8ZD Dorchester, Dorset, UK |
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Abstract: | From 2000–2003 a range of Farm Scale Evaluation (FSE) trials were established in the UK to assess the effect of the release
and management of herbicide tolerant (HT) crops on the abundance and diversity of farmland wildlife compared with their conventionally
managed non-GM-equivalents. The objective of this research project was to investigate gene flow within the winter (WOSR) and
spring oilseed rape (SOSR) FSE trials and to develop a statistical model for the prediction of cross-pollination frequency
that can be used to evaluate current separation distance guidelines. Seed samples were collected from the non-GM half of the
trial sites and were tested for evidence of cross-pollination from the GM HT halves using a quantitative PCR assay specific
to the HT (bar) gene. Rates of cross-pollination were found to decrease rapidly with increasing distance from the GM source. The quantitative
data were subjected to statistical analysis and a two-step model was found to provide the best fit for the data. Significant
differences were found between the results for WOSR, SOSR and varietal association (VA) crops. The model predicted that the
%GM content (including upper 95% confidence limits) of a sample taken at a distance of 50 m away from the GM source would
be 0.04% (0.84%) for WOSR, 0.02% (0.39%) for SOSR, 0.77% (21.72%) for WOSR VA and 0.37% (5.18%) for SOSR VA. The data and
models presented here are discussed in the context of necessary separation distances to meet various possible thresholds for
adventitious presence of GM in OSR.
The British Crown's right to retain a non-exclusive, royalty-free licence in and to any copyright is acknowledged. |
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Keywords: | Brassica napus gene flow herbicide tolerant oilseed rape real time PCR transgenic crops |
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