Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments |
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Authors: | Michael Salzmann Gerhard Wider Konstantin Pervushin Kurt Wüthrich |
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Affiliation: | (1) Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, USA |
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Abstract: | In experiments with proteins of molecular weights around 100 kDa the implementation of [15N,1H]-TROSY-elements in [15N]-constant-time triple resonance experiments yields sensitivity enhancements of one to two orders of magnitude. An additional gain of 10 to 20% may be obtained with the use of sensitivity enhancement elements. This paper describes a novel sensitivity enhancement scheme which is based on concatenation of the 13 C 15N magnetization transfer with the ST2-PT element, and which enables proper TROSY selection of the 15N multiplet components. |
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Keywords: | concatenation protein NMR resonance assignments sensitivity enhancement TROSY in triple resonance experiments |
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