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| 弗氏志贺菌磷酸化蛋白的检测 | |
| 引用本文： | 刘小青,朱力,吴松羽,商娜,冯尔玲,魏华,王恒樑. 弗氏志贺菌磷酸化蛋白的检测[J]. 生物技术通讯, 2010, 21(5): 691-694. DOI: 10.3969/j.issn.1009-0002.2010.05.021 |
| 作者姓名： | 刘小青 朱力 吴松羽 商娜 冯尔玲 魏华 王恒樑 |
| 作者单位： | 1. 军事医学科学院,生物工程研究所,病原微生物生物安全国家重点实验室,北京,100071;南昌大学,食品科学与技术国家重点实验室,江西,南昌,330047 2. 军事医学科学院,生物工程研究所,病原微生物生物安全国家重点实验室,北京,100071 3. 南昌大学,食品科学与技术国家重点实验室,江西,南昌,330047 |
| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划 |
| 摘 要： | 目的:检测弗氏志贺菌全菌蛋白中被磷酸化修饰的蛋白。方法:制取弗氏2a志贺菌2457T野生株全菌磷酸化蛋白样品时加入磷酸化酶抑制剂,随后对样品进行双向电泳,以抗磷酸丝氨酸/苏氨酸/酪氨酸抗体为免疫探针,通过Western印迹找到被磷酸化的蛋白,并进行胶内酶解及MALDI-TOF质谱分析。结果与结论:共检测到13个磷酸化蛋白,其中9个为代谢途径中的酶。 |
| 关 键 词： | 弗氏志贺菌 磷酸化蛋白 检测 |
Detection of Phosphorylated Proteins of Shigella flexneri |
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| LIU Xiao-qing,ZHU Li,WU Song-Yu,SHANG Na,FENG Er-Ling,WEI Hua,WANG Heng-Liang. Detection of Phosphorylated Proteins of Shigella flexneri[J]. Letters in Biotechnology, 2010, 21(5): 691-694. DOI: 10.3969/j.issn.1009-0002.2010.05.021 | |
| Authors: | LIU Xiao-qing ZHU Li WU Song-Yu SHANG Na FENG Er-Ling WEI Hua WANG Heng-Liang |
| Affiliation: | Liang1 1.State Key Laboratory of Pathogen and Biosecurity,Beijing Insitute of Biotechnology,Beijing 100071;2.State Key Laboratory of Food Science,Nanchang University,Nanchang 330047;China |
| Abstract: | Objective： To detect phosphorylated proteins of Shigella flexneri.Methods： Whole-cell proteins of wild-type S.flexneri 2a 2457T treated with phosphatase inhibitor were separated by two-dimensional electrophoresis.One of the gels was used for Western blotting to detect phosphorylated proteins.The corresponding immunogenicity spots found on the other Coomassie brilliant blue stained gel were cut out,digested by trypsin and analyzed by MALDI-TOF MS.Results Conclusion： Thirteen phosphorylated proteins were detected,and 9 of them are enzymes involved in metabolic pathway. |
| Keywords: | Shigella flexneri phosphorylated proteins detection |
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