In vitro induction and expression of interleukin 2 receptor in a clonal T helper cell differentiation model |
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Authors: | G Bismuth L Leclercq M Duphot J L Moreau J Theze |
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Affiliation: | Unité d''Immunogénétique Cellulaire, Département d''Immunologie, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, France |
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Abstract: | Induction and expression of interleukin 2 (IL 2) receptor have been studied using a poly( Glu60 Ala30 Tyr10 ) (GAT)-specific T cell clone of mouse origin. This clone (52-3) has been characterized and it exhibits functional properties of T helper (TH) cells: it leads to a specific anti-DNP response in the presence of DNP-GAT and DNP-primed B cells and it secretes biological activities which can induce polyclonal B cell proliferation and IgM secretion. In vitro this clone mimics the activation stages of normal T lymphocytes and can be obtained under two states of differentiation. depending on the antigen-specific signal provided by antigen-presenting cells (APC). The expression of IL 2 receptor by this clone has been studied by (i) its response to IL 2, (ii) its capacity to absorb IL 2 bioactivity, and (iii) its reactivity with monoclonal antibody 7D4 specific for mouse IL 2 receptor. All the results indicate that the unstimulated state does not express the IL 2 receptor while the activated state does. Clone 52-3 has been compared with clone 14-1.6 that derives from a TH cell line and expresses the IL 2 receptor constitutively. 52-3 offers a good experimental model for studying in vitro, in a clonal TH cell population, the detailed mechanism of IL 2 receptor induction. |
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Keywords: | All correspondence should be addressed to Dr. Jacques Theze Unité d'Immunogénétique Cellulaire Département d'Immunologie Institut Pasteur 25 rue du Docteur Roux F-75724 Paris Cédex 15 France. |
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