Selective expression of light-harvesting complexes alters phospholipid composition in the intracytoplasmic membrane and core complex of purple phototrophic bacteria |
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| Institution: | 1. Faculty of Science, Ibaraki University, Mito 310-8512, Japan;2. Research Institute for Integrated Science, Kanagawa University, 3-27-1 Rokkakubashi, Kanagawa-ku, Yokohama, Kanagawa 221-8686, Japan;3. National Institute of Technology, Ariake College, Omuta, Fukuoka 836-8585, Japan;4. Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;5. School of Biological Sciences, Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA;6. Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada, Kobe 657-8501, Japan;1. Life Science Research Infrastructure Group, RIKEN SPring-8 Center, Kouto, Hyogo, Japan;2. Laboratory for Protein Crystallography, Institute for Protein Research, Osaka University, Suita, Osaka, Japan;3. Faculty of Science, Department of Science, Yamagata University, Yamagata, Japan;4. National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Japan;5. Chemistry Research Laboratory, South Parks Road, Oxford University, United Kingdom;6. Cellular and Structural Physiology Institute, Nagoya University, Nagoya, Japan;7. Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Japan;8. Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, Ibaraki, Osaka, Japan;9. Department of Basic Biology, School of Life Science, the Graduate University for Advanced Studies, Sokendai, Okazaki, Japan;1. Institute of Physics, University of Tartu, W. Ostwald Str. 1, 50411 Tartu, Estonia;2. Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia;1. Department of Biophysics, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands;2. Faculty of Science, Ibaraki University, Mito, Ibaraki 310-8512, Japan |
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| Abstract: | Phospholipid–protein interactions play important roles in regulating the function and morphology of photosynthetic membranes in purple phototrophic bacteria. Here, we characterize the phospholipid composition of intracytoplasmic membrane (ICM) from Rhodobacter (Rba.) sphaeroides that has been genetically altered to selectively express light-harvesting (LH) complexes. In the mutant strain (DP2) that lacks a peripheral light-harvesting (LH2) complex, the phospholipid composition was significantly different from that of the wild-type strain; strain DP2 showed a marked decrease in phosphatidylglycerol (PG) and large increases in cardiolipin (CL) and phosphatidylcholine (PC) indicating preferential interactions between the complexes and specific phospholipids. Substitution of the core light-harvesting (LH1) complex of Rba. sphaeroides strain DP2 with that from the purple sulfur bacterium Thermochromatium tepidum further altered the phospholipid composition, with substantial increases in PG and PE and decreases in CL and PC, indicating that the phospholipids incorporated into the ICM depend on the nature of the LH1 complex expressed. Purified LH1–reaction center core complexes (LH1–RC) from the selectively expressing strains also contained different phospholipid compositions than did core complexes from their corresponding wild-type strains, suggesting different patterns of phospholipid association between the selectively expressed LH1–RC complexes and those purified from native strains. Effects of carotenoids on the phospholipid composition were also investigated using carotenoid-suppressed cells and carotenoid-deficient species. The findings are discussed in relation to ICM morphology and specific LH complex–phospholipid interactions. |
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