Trends in Extended Spectrum Beta-Lactamase (ESBL) Producing Enterobacteriaceae and ESBL Genes in a Dutch Teaching Hospital,Measured in 5 Yearly Point Prevalence Surveys (2010-2014) |
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| Authors: | Ina Willemsen Stijn Oome Carlo Verhulst Annika Pettersson Kees Verduin Jan Kluytmans |
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| Affiliation: | 1. Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.; 2. Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.; 3. Julius Center for Health Sciences and Primary Care, UMC Utrecht, Utrecht, the Netherlands.; University of Cambridge, UNITED KINGDOM, |
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| Abstract: | This paper describes the trends in prevalence of ESBL producing Enterobacteriaceae (ESBL-E) and ESBL genes, measured in five consecutive yearly Point Prevalence Surveys (PPS). All patients present in the hospital and in a day-care clinic (including patients on dialysis) on the day of the survey, were screened for perianal ESBL-E carriage. Perianal swabs were taken and cultured using an enrichment broth and a selective agar plate. Both phenotypic and genotypic methods were used to detect the production of ESBL, presence of ESBL-genes and clonal relatedness. Out of 2,695 patients, 135 (5.0%) were tested ESBL-E positive. The overall ESBL-E prevalence was stable over the years. Overall 5.2% of all ESBL-E were acquired by nosocomial transmission. A relative decrease of CTX-M-1-1-like ESBL genes (from 44 to 25%, p = 0.026) was observed, possibly related to the strong (>60%) decrease in antibiotic use in livestock in our country during the same period. |
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