Identification of Nd1, a novel murine kelch family protein,involved in stabilization of actin filaments |
| |
Authors: | Sasagawa Kazushi Matsudo Yuji Kang Myenmo Fujimura Lisa Iitsuka Yoshinori Okada Seiji Ochiai Takenori Tokuhisa Takeshi Hatano Masahiko |
| |
Institution: | Department of Developmental Genetics (H2), Graduate School of Medicine, Chiba University, Japan. |
| |
Abstract: | We isolated Nd1, a novel kelch family gene that encodes two forms of proteins, Nd1-L and Nd1-S. Nd1-L contains a BTB/POZ domain in its N terminus and six kelch repeats in the C terminus. Nd1-S has the BTB/POZ domain but lacks the six kelch repeats. Nd1-L but not Nd1-S mRNA is detected ubiquitously in normal mouse tissues. Nd1-L and Nd1-S proteins can form a dimer through the BTB/POZ domain. Nd1-L colocalizes with actin filaments detected using a confocal microscope, and its kelch repeats bind to them in vitro. Overexpression of Nd1-L in NIH3T3 cells delayed cell growth by affecting the transition of cytokinesis. Furthermore, the overexpression prevented NIH3T3 cells from cell death induced by actin destabilization but not by microtubule dysfunction. These data suggest that Nd1-L functions as a stabilizer of actin filaments as an actin-binding protein and may play a role in the dynamic organization of the actin cytoskeleton. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|